Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
生物su化番茄凝集su
產品標簽
番茄凝集su（Tomato lectin）；FITC-LEL；Intravascular perfusion 血管內灌注；Lectin perfusion 凝集su灌注；Microvasculature 微血管；Endothelial cells 內皮細胞；
產品信息【，現貨供應，大量引用文獻支持】
產品名稱 | 產品編號 | 規格 | 目錄價（元） |
Biotin labeled Lycopersicon Esculentum (Tomato) Lectin | B-1175 | 1mg | 2541 |
產品描述
番茄凝集su（Tomato lectin，Lycopersicon Esculentum Lectin（LEL）），是一種非常穩定的單亞基糖蛋白，約含有等量的蛋白質和碳水化合su（DSL）和麥胚凝集su（WGA）有部分相同的特異性，但在許多多面呈現出*之處。番茄凝集suLEL能夠很好地與血型糖蛋白（glycophorin）和Tamm-Horsfall糖蛋白結合，能夠有效結合和標記嚙齒類動物的血管內皮（vascular endothelium）。
產品特征
Synonyms | Biotin-LEL; Biotin-LEA; |
Sugar Specificity | [GlcNAc]1-3, N-Acetylglucosamine |
Applications | Immunohistochemistry / Immunocytochemistry, Elispot, ELISAs, Glycobiology, Immunofluorescence, Blotting Applications |
Working Concentration | For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
State | Powder |
Reconstitution | Reconstitute by adding 0.5 ml of water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca++. |
Inhibiting/Eluting Sugar | Chitin Hydrolysate |
Storage | Refrigerate; for long term storage, aliquots may be stored frozen. |
使用方法（Histochemistry）【更多請點擊此處下載Lectin Immunohistochemistry, Elisa, Western Blots Guidelines】
1a. Staining procedure for paraffin sections: Deparaffinize and hydrate tissue sections through xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water. If required, retrieve antigens using the Antigen Unmasking Solution (H-3300 or H-3301).
1b. Staining procedure for frozen sections: Air dry sections. Immediay before staining, fix sections with acetone. Transfer slices to buffer. If endogenous enzyme activities are present, inactivate using appropriate methods.
2. Perform Streptavidin/Biotin blocking if required following kit instructions (SP-2002). Avidin in the Avidin/Biotin Blocking Kit (SP-2001) is a glycoprotein containing terminal mannose residues. Use of the Avidin/Biotin Blocking Kit is not recommended especially when using mannose-specific lectins.
3. Block non-specific binding by incubating section with Carbo-Free™ Blocking Solution (SP-5040) for 30 minutes at room temperature. Blot excess blocking solution from the sections.
4. Apply biotinylated lectin at approximay 2-20 µg/ml in PBS (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature. Wash with TPBS (PBS + 0.05% Tween™ 20).
5. Prepare VECTASTAIN® ® ABC (peroxidase, PK-6100) or VECTASTAIN® ABC-AP (alkaline phosphatase, AK-5000) reagents according to the kit instructions. Apply to the sections and incubate for 30 minutes at room temperature. Wash with TPBS.
6. Apply an appropriate precipitating substrate for the enzyme system used in step 4. For peroxidase, ImmPACT™ DAB (SK-4105) is recommended; for alkaline phosphatase, ImmPACT™ Vector® Red (SK-5105). Rinse in tap water.
7. Counterstain (optional), clear and mount. For galactose or GalNAc-specific lectins we recommend that sections be mounted in VECTASHIELD® HardFSet™ Mounting Medium (H-1400) or VECTASHIELD® HardFSet™ Mounting Medium with DAPI (H-1500).
相關產品：
產品名稱 | 產品編號 | 規格 | 目錄價（元） |
Fluorescein labeled Lycopersicon Esculentum (Tomato) Lectin （Ex/Em=495/515nm，綠色熒光） | FL-1171 | 1mg | 3331 |
Texas Red labeled Lycopersicon Esculentum (Tomato) Lectin （Ex/Em=595/615nm，紅色熒光） | TL-1176 | 1mg | 3501 |
DyLight 488 labeled Lycopersicon Esculentum (Tomato) Lectin （Ex/Em=493/518nm，綠色熒光） | DL-1174 | 1mg | 3288 |
DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin （Ex/Em=593/618nm，紅色熒光） | DL-1177 | 1mg | 3288 |
Biotin labeled Lycopersicon Esculentum (Tomato) Lectin | B-1175 | 1mg | 2541 |
Unconjugated Lycopersicon Esculentum (Tomato) Lectin | L-1170 | 2mg | 3352 |
Chitin Hydrolysate | SP-0090 | 10ml | 1900 |
引用文獻【Vector Labs，Tomato Lectin】
— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。
