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Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Growth hormone (GH), a polypeptide hormone with a molecular weight of 23000 Daltons released from somatotropes of the anterior pituitary, is regulated by several neurotransmitters and neuropeptides. Among other functions it plays an essential role in regulating body growth.
生長(zhǎng)激素 (GH) 是一種分子量為 23000 道爾頓的多肽激素,從垂體前葉的體細(xì)胞中釋放出來(lái),由幾種神經(jīng)遞質(zhì)和神經(jīng)肽調(diào)節(jié)。除其他功能外,它在調(diào)節(jié)身體生長(zhǎng)方面起著至關(guān)重要的作用。
艾美捷Bertin Bioreagent艾美捷Bertin Bioreagent生長(zhǎng)激素(GH)大鼠ELISA kit#A05104
規(guī)格 96 孔
航運(yùn) 干冰
特 異性
大鼠生長(zhǎng)激素:100%
小鼠生長(zhǎng)激素:91%
大鼠催乳素:<1%
大鼠FSH:<0.1%
人類生長(zhǎng)激素:<0.1%
大鼠 LH: <0.1%
大鼠TSH:<0.1%
應(yīng)用介質(zhì) 血漿和培養(yǎng)上清液|無(wú)需提取
樣品體積 50 微升
示 蹤 乙酰膽-堿酯酶AChE
檢測(cè)限 0.04 納克/毫升
標(biāo)準(zhǔn)曲線范圍 0.25-25 納克/毫升
Bertin Bioreagent酰化生長(zhǎng)素釋放肽豬ELISA kit文獻(xiàn)參考:
PRODUCT EXPLANATION: GROWTH HORMONE KIT DEVELOPMENT
Ezan E., Laplante E., Bluet-Pajot M.-T. et al. An Enzyme Immunoassay for rat growth hormone: validation and application to the determination of plasma levels and in vitro release. J. Immunoassay, 18(4), 335-336, 1997
Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877
European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011
Bertin Bioreagent致力于研發(fā)與炎癥,氧化損傷,內(nèi)分泌學(xué),糖尿病,肥胖癥,高血壓,精神藥理學(xué),癌癥,哮喘,朊病毒疾病,藥代動(dòng)力學(xué)和代謝等相關(guān)的化學(xué)試劑 ,蛋白,底物,抗體及檢測(cè)試劑盒等。艾美捷科技是Bertin Bioreagent的中國(guó)代理商,為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。
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